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实时荧光定量PCR 2

ATP荧光检测 1

COVID-19 1

DNA计算 1

LM331型电压-频率转换器 1

PCR核酸检测 1

Visual Basic 6.0 1

cDNA克隆 1

串行通信 1

促黄体激素（LH） 1

分子诊断 1

半滑舌鳎 1

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

《医学前沿（英文）》 2010年第4卷第2期页码 204-207 doi: 10.1007/s11684-010-0043-4

摘要： Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

关键词： Angiostrongylus cantonensis Pomacea canaliculata multiplex polymerase chain reaction diagnosis pooling

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Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical stool specimens

null

《医学前沿（英文）》 2018年第12卷第2期页码 196-205 doi: 10.1007/s11684-017-0560-5

摘要：

We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x² = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.

关键词： Clostridium difficile multiplex PCR capillary electrophoresis detection characterization

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Arthrogryposis multiplex congenita: classification, diagnosis, perioperative care, and anesthesia

null

《医学前沿（英文）》 2017年第11卷第1期页码 48-52 doi: 10.1007/s11684-017-0500-4

摘要：

Arthrogryposis multiplex congenita (AMC) is a rare disorder characterized by non-progressive, multiple contractures. In addition to affected extremities, patients may also present microstomia, decreased temporomandibular joint mobility. Although the etiology of AMC is unclear, any factor that decreases fetal movement is responsible for AMC. Thus, accurate diagnosis and classification are crucial to the appropriate treatment of AMC. The development of ultrasound technology has enabled prenatal diagnosis. Very early treatment is favorable, and multidisciplinary treatment is necessary to improve the function of AMC patients. Most patients require surgery to release contracture and reconstruct joints. However, perioperative care is challenging, and difficult airway is the first concern of anesthesiologists. Postoperative pulmonary complications are common and regional anesthesia is recommended for postoperative analgesia. This review on AMC is intended for anesthesiologists. Thus, we discuss the treatment and perioperative management of patients undergoing surgery, as well as the diagnosis and classification of AMC.

关键词： arthrogryposis amyoplasia distal arthrogryposis anesthesia

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Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

《环境科学与工程前沿（英文）》 2011年第5卷第1期页码 21-27 doi: 10.1007/s11783-011-0303-9

摘要： New high-throughput technologies continue to emerge for studying complex microbial communities. In particular, massively parallel pyrosequencing enables very high numbers of sequences, providing a more complete view of community structures and a more accurate inference of the functions than has been possible just a few years ago. In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring of specific community members over time, space, or different environmental conditions. In this review, the principles of these two methods and their complementary applications in studying microbial ecology in bioenvironmental systems are discussed. The parallel sequencing of amplicon libraries and using barcodes to differentiate multiple samples in a pyrosequencing run are explained. The best procedures and chemistries for QPCR amplifications are also described and advantages of applying automation to increase accuracy are addressed. Three examples in which pyrosequencing and QPCR were used together to define and quantify members of microbial communities are provided: in the human large intestine, in a methanogenic digester whose sludge was made more bioavailable by a high-voltage pretreatment, and on the biofilm anode of a microbial electrolytic cell. The key findings in these systems and how both methods were used in concert to achieve those findings are highlighted.

关键词： polymerase chain reaction (PCR) microbial communities pyrosequencing gut microbial fuel cell sludge

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低成本PCR微反应器阵列的温度测量与控制技术

李现明,张玉林,李建田

《中国工程科学》 2007年第9卷第7期页码 76-81

摘要：

设计了一种用于聚合酶链式反应的低成本微反应器，其内部无微加热器、微传感器。针对这种低成本的微反应器，提出了一种温度测量与控制方案，其特点是将各反应器温度的集中控制与分散控制有机结合，能够同时完成上百只微反应器快速、准确的温度循环。重点阐述了温度传感器负荷效应的提取与补偿、动态误差补偿、微反应器温度间接测量误差评定、微反应器温度串级控制策略。

关键词：微反应器测量控制聚合酶链式反应

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Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

《环境科学与工程前沿（英文）》 2011年第5卷第3期页码 488-488 doi: 10.1007/s11783-011-0342-2

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Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan

《化学科学与工程前沿（英文）》 2018年第12卷第4期页码 806-814 doi: 10.1007/s11705-018-1739-2

摘要：

The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.

关键词： synthetic biology genome rearrangement prodeoxyviolacein SCRaMbLE Saccharomyces cerevisiae

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Safety evaluation of microbial pesticide (HaNPV) based on PCR method

Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian

《化学科学与工程前沿（英文）》 2019年第13卷第2期页码 377-384 doi: 10.1007/s11705-018-1777-9

摘要： Microbial pesticides can prevent and control diseases and pests of crops, and has become one of the important measures to ensure food and environmental safety. However, the potential harm of microbial pesticides to humans and animals is a serious concern at home and abroad. In this paper, we have investigated the infectivity and pathogenicity of a representative of viral microbial pesticides, helicoverpa armigera nuclear polyhedrosis virus (HaNPV), by specific and highly sensitive polymerase chain reaction technology. The results show that HaNPV can be gradually cleared in a short time after getting into blood of experimental rats, and does not infect other tissues or organs of animals; also indicate that the test subjects are not infectious to experimental rats after intravenous injection of HaNPV. Our method has good specificity and repeatability, and could provide an important reference for establishment of infectivity and pathogenicity detection methods for viral microbial pesticides in future.

关键词： microbial pesticides HaNPV acute intravenous injection infectivity pathogenicity

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Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker Erratum

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

《工程（英文）》 2022年第18卷第11期页码 259-259 doi: 10.1016/j.eng.2022.09.002

关键词： online THE AUTHORS.Published repertoire behalf Engineering September genotyping Academy HD-marker approach” Education Press Limited Company.

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生物检测及分子诊断高端装备的研制及应用

彭年才,李磊,李政,苗保刚,李明,赵垚,蒋庄德

《中国工程科学》 2013年第15卷第1期页码 57-62

摘要：

以生物检测与分子诊断技术发展为背景，总结了团队在医学诊断、疾控、食品安全、检疫检验、生物学医学科学研究等领域的分子诊断高端装备的研制情况，重点介绍了实时荧光定量聚合酶链式反应（PCR）分子诊断仪关键技术的研发及仪器工程化技术

关键词：生物检测分子诊断实时荧光定量PCR ATP荧光检测

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基于抗原和聚合酶链式反应的组合策略对模拟社区内遏制COVID-19传播效果的定量分析 Article

黄蔷如, 孙艳侠, 贾萌萌, 张婷, 陈方圆, 姜明月, 王晴, 冯录召, 杨维中

《工程（英文）》 2023年第28卷第9期页码 234-242 doi: 10.1016/j.eng.2023.01.004

摘要：研究结果表明抗原检测及其组合策略比聚合酶链式反应（polymerase chain reaction, PCR）相关策略更高效，更有助于降低医院床位和重症监护病房床位需求。较短的结果返回时间是抗原检测策略的主要优势所在，也是优化PCR策略的关键因素。优化调整检测结果返回时间后，低频次的检测策略的效果可以媲美每日连续检测策略的效果。

关键词： COVID-19 抗原检测 PCR核酸检测检测策略

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半滑舌鳎促黄体激素基因克隆和表达分析及其血清浓度测定

柳学周,史宝,王珊珊,徐永江,李晓晓

《中国工程科学》 2014年第16卷第9期页码 50-60

摘要：实时荧光定量组织表达分析表明，LH mRNA在脑、垂体、卵巢等组织中表达，垂体中表达量最丰富，其他组织尤以脑、性腺表达量较高，推测半滑舌鳎LH可能具有广泛的垂体外生理功能；实时荧光定量PCR方法对繁殖周期雌性半滑舌鳎

关键词：半滑舌鳎促黄体激素（LH） cDNA克隆实时荧光定量PCR 放射免疫测定

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基于V/F的智能多路数据采集系统及串口通信

贾贵玺,李洪凤,齐炜

《中国工程科学》 2004年第6卷第11期页码 58-62

摘要：

智能多路数据采集系统实现了多路温度采集与检测，利用仪用放大器AD620进行信号放大，采用LM331型电压-频率转换器进行电量转换，采用89S52单片机进行数据采集及存储并用Visual Basic 6.0实现单片机与PC机的通信；最后给出了智能多路数据采集系统在梅江小区综合办公楼生态能源示范系统的应用数据。

关键词： LM331型电压-频率转换器 Visual Basic 6.0 串行通信

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Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China

Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI

《环境科学与工程前沿（英文）》 2014年第8卷第5期页码 693-702 doi: 10.1007/s11783-014-0635-3

摘要： As low oxygen and high ultraviolet (UV) exposure might significantly affect the microbial existence in plateau, it could lead to a specialized microbial community. To determine the abundance and distribution of ammonia-oxidizing archaea (AOA) in agricultural soil of plateau, seven soil samples were collected respectively from farmlands in Tibet and Yunnan cultivating the wheat, highland-barley, and colza, which are located at altitudes of 3200–3800 m above sea level. Quantitative PCR (q-PCR) and clone library targeting on gene were used to quantify the abundances of AOA and ammonia-oxidizing bacteria (AOB), and characterize the community structures of AOA in the samples. The number of AOA cells (9.34 × 10 –2.32 × 10 g soil) was 3.86–21.84 times greater than that of AOB cells (6.91 × 10 –1.24 × 10 g soil) in most of the samples, except a soil sample cultivating highland-barley with an AOA/AOB ratio of 0.90. Based Kendall’s correlation coefficient, no remarkable correlation between AOA abundance and the environmental factor was observed. Additionally, the diversities of AOA community were affected by total nitrogen and organic matter concentration in soils, suggesting that AOA was probably sensitive to several environmental factors, and could adjust its community structure to adapt to the environmental variation while maintaining its abundance.

关键词： ammonia-oxidizing archaea ammonia-oxidizing bacteria quantitative PCR clone library plateau

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基于多层网络随机块模型的多重社交关系量化方法 Science Letter

吴旻诚,李振,邵存祺,贺诗波

《信息与电子工程前沿（英文）》 2021年第22卷第11期页码 1458-1462 doi: 10.1631/FITEE.2000617

摘要：在线社交网络使世界各地的人们能够方便地建立各种社交关系，受到极大关注。但是，观测到的社交网络结构往往是多种社交关系的聚合结构。因此，通过观测到的单层结构完整地重构真实网络的多重结构非常重要。本文通过多层网络随机块模型描述多重社交关系，其中不同层对应不同属性（例如，社交网络用户的年龄和性别）。本文旨在利用最大似然估计提高模型参数估计精度，其中估计精度由数据和模型参数之间的交叉熵定义。在本文中，多重网络中每一层节点的分类由其自然属性决定，并且假设多重网络的单层聚合结构已知。由于原多重网络具有较高自由度，因此通过添加一个独立的功能层增加模型参数，以充分覆盖自由度，并在理论上获得功能层的最佳分块数。最后，通过仿真实验，从链接概率误差、交叉熵、接收者操作特征曲线以及贝叶斯因子4个角度验证了本文方法的有效性。

关键词：社交网络；多重网络；随机块模型

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标题作者时间类型操作

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

期刊论文

Simultaneous detection and characterization of toxigenic：Clostridium difficile directly from clinical stool specimens

null

期刊论文

Arthrogryposis multiplex congenita: classification, diagnosis, perioperative care, and anesthesia

null

期刊论文